Recombinant Human immunodeficiency virus type 1 group M subtype G Protein Vpr (vpr), Human immunodeficiency virus type 1 group M subtype G (isolate SE6165) (HIV-1), E.coli, N-terminal 6xHis-KSI-tagged, 26.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human immunodeficiency virus type 1 group M subtype G Protein Vpr (vpr)

Recombinant Human immunodeficiency virus type 1 group M subtype G Protein Vpr (vpr) — During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. Purity >90%.

SKU: BCRECP-004742 Categories: ,
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Product Specifications

Product SkuBCRECP-004742
Product DescriptionRecombinant Human immunodeficiency virus type 1 group M subtype G Protein Vpr (vpr) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 1-96aa. [Accession | O89942].
Uniprot No.O89942
Gene Namesvpr
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-96aa
SpeciesHuman immunodeficiency virus type 1 group M subtype G (isolate SE6165) (HIV-1)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight26.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions.

Biological Context

Subcellular Location: Virion; Host nucleus; Host extracellular space

Product Specifications

Recombinant Human immunodeficiency virus type 1 group M subtype G Protein Vpr (vpr) is a recombinant protein from Human immunodeficiency virus type 1 group M subtype G (isolate SE6165) (HIV-1), expressed in E.coli, covering amino acids 1-96aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 26.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions

Subcellular Location

Virion; Host nucleus; Host extracellular space

Subunit

Homooligomer, may form homodimer. Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C-terminus of the P6gag protein. Interacts with host UNG. May interact with host RAD23A/HHR23A. Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediating ubiquitination of host proteins such as TERT and ZGPAT and arrest of the cell cycle in G2 phase

Gene: vpr  |  Organism: Human immunodeficiency virus type 1 group M subtype G (isolate SE6165)  |  Synonyms: R ORF protein; Viral protein R

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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