Recombinant Human Interleukin-18 receptor accessory protein (IL18RAP), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 39.8 kDa, purity >95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
,

Recombinant Human Interleukin-18 receptor accessory protein (IL18RAP), partial (Active)

Recombinant Human Interleukin-18 receptor accessory protein (IL18RAP), partial (Active) — Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation. Purity >95%.

SKU: BCACP-000610 Categories: ,
Online Inquiry →
Product SKUBCACP-000610
Product DescriptionRecombinant Human Interleukin-18 receptor accessory protein (IL18RAP) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 20-356aa. [Accession | O95256].
Uniprot No.O95256
Gene NamesIL18RAP
PurityGreater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region20-356aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight39.8 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human EPCAM at 2 μg/mL can bind Anti-EPCAM recombinant antibody . The EC50 is 1.098-1.401 ng/mL.;EPCAM Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human EPCAM with an affinity constant of 0.742 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesInterleukin-18 receptor accessory protein; IL-18 receptor accessory protein; IL-18RAcP; EC 3.2.2.6; Accessory protein-like; AcPL; CD218 antigen-like family member B; CDw218b; IL-1R accessory protein-like; IL-1RAcPL; Interleukin-1 receptor 7; IL-1R-7; IL-1R7; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; IL-18R-beta; IL-18Rbeta; CD antigen CD218b

Function

Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation. May play a role in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (Probable).

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Detected in adrenal gland, bone marrow, brain, fetal brain, fetal liver, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, prostate, salivary gland, skeletal muscle, spinal cord, testis, thymus, thyroid, trachea and uterus. Strongly expressed in peripheral blood leukocytes and spleen and, to a lesser extent, in colon. Specifically coexpressed with IL18R1 in T-helper 1 (Th1)cells

Product Specifications

Recombinant Human Interleukin-18 receptor accessory protein (IL18RAP), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 20-356aa, with C-terminal 10xHis-tagged tag, molecular weight 39.8 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation (PubMed:14528293, PubMed:25500532, PubMed:9792649). May play a role in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (Probable)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Detected in adrenal gland, bone marrow, brain, fetal brain, fetal liver, heart, kidney, lung, liver, peripheral blood leukocytes, placenta, prostate, salivary gland, skeletal muscle, spinal cord, testis, thymus, thyroid, trachea and uterus (PubMed:17897836). Strongly expressed in peripheral blood leukocytes and spleen and, to a lesser extent, in colon (PubMed:9792649). Specifically coexpressed with IL18R1 in T-helper 1 (Th1)cells (PubMed:10653850, PubMed:10925275, PubMed:11046021)

Subunit

Forms a ternary complex with IL18 and IL18R1 (PubMed:14528293, PubMed:25500532). Within this complex, IL18R1 is involved in ligand-binding and IL18RAP in signaling leading to NF-kappa-B and JNK activation (Probable)

Gene: IL18RAP  |  Organism: Homo sapiens  |  Synonyms: Accessory protein-like; CD218 antigen-like family member B; CDw218b; IL-1R accessory protein-like; Interleukin-1 receptor 7; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top