Recombinant Human Intestinal-type alkaline phosphatase (ALPI) (Active) — recombinant protein, expressed in Mammalian cell. Purity >95%.
| Product SKU | BCACP-000179 |
| Product Description | Recombinant Human Intestinal-type alkaline phosphatase (ALPI) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 20-503aa. [Accession | P09923]. |
| Uniprot No. | P09923 |
| Gene Names | ALPI |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Expression System | Mammalian cell |
| Expression Region | 20-503aa |
| Species | Homo sapiens (Human) |
| Tag Info | N-terminal 10xHis-tagged |
| Molecular weight | 55.2 kDa |
| Activity | After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies , cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells . The ED50 is 0.3708-0.5608 μg/ml.;After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies , cytotoxicity experiments were conducted on CT26/Human CCR8 cells . The ED50 is 0.4661-0.8614 μg/ml. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | (IAP)(Intestinal alkaline phosphatase) |
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Recombinant Human Intestinal-type alkaline phosphatase (ALPI) (Active) is a recombinant protein, expressed in Mammalian cell, covering amino acids 20-503aa, with N-terminal 10xHis-tagged tag, molecular weight 55.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications. Explore more Enzyme proteins →
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies , cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells . The ED50 is 0.3708-0.5608 μg/ml.;After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies , cytotoxicity experiments were conducted on CT26/Human CCR8 cells . The ED50 is 0.4661-0.8614 μg/ml.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
Alkaline phosphatase that can hydrolyze various phosphate compounds
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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
