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Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) (Active)

Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) (Active) — recombinant protein from Rattus norvegicus (Rat), expressed in Mammalian cell. Purity >95%.

SKU: BCACP-000181 Categories: ,
Product SKUBCACP-000181
Product DescriptionRecombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 21-511aa. [Accession | P15693].
Uniprot No.P15693
Gene NamesAlpi
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region21-511aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 10xHis-tagged
Molecular weight55.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Mouse Ttr at 5 μg/mL can bind Mouse Rbp4, the EC50 is 38.07-75.83 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(IAP-1)(Intestinal alkaline phosphatase 1)(Intestinal alkaline phosphatase I)(IAP-I)

Function

Alkaline phosphatase that can hydrolyze various phosphate compounds.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor)

Product Specifications

Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) (Active) is a recombinant protein from Rattus norvegicus (Rat), expressed in Mammalian cell, covering amino acids 21-511aa, with N-terminal 10xHis-tagged tag, molecular weight 55.9 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Ttr at 5 μg/mL can bind Mouse Rbp4, the EC50 is 38.07-75.83 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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