Recombinant Human Isoleucine--tRNA ligase, cytoplasmic (IARS), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 26.3kDa, purity >90% as determined by SDS-PAGE.
,

Recombinant Human Isoleucine–tRNA ligase, cytoplasmic (IARS), partial

Recombinant Human Isoleucine–tRNA ligase, cytoplasmic (IARS), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >90%.

SKU: BCRECP-000878 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-000878
Product DescriptionRecombinant Human Isoleucine--tRNA ligase, cytoplasmic (IARS) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 693-852aa. [Accession | P41252].
Uniprot No.P41252
Gene NamesIARS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region693-852aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight26.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Biological Context

Subcellular Location: Cytoplasm; Cytoplasm, cytosol
Tissue Specificity: Expressed in liver and muscle (at protein level)
Disease Association: Growth retardation, impaired intellectual development, hypotonia, and hepatopathy (GRIDHH) : An autosomal recessive disorder characterized by severe growth retardation with prenatal onset, intellectual disability, muscular hypotonia, and hepatic dysfunction. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Isoleucine–tRNA ligase, cytoplasmic (IARS), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 693-852aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 26.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA

Subcellular Location

Cytoplasm; Cytoplasm, cytosol

Disease Association

Growth retardation, impaired intellectual development, hypotonia, and hepatopathy (GRIDHH) : An autosomal recessive disorder characterized by severe growth retardation with prenatal onset, intellectual disability, muscular hypotonia, and hepatic dysfunction. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed in liver and muscle (at protein level)

Subunit

Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:19131329, PubMed:19289464)

Gene: IARS1  |  Organism: Homo sapiens  |  Synonyms: Isoleucyl-tRNA synthetase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top