Recombinant Human Keratin, type II cytoskeletal 4 (KRT4), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 73.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Keratin, type II cytoskeletal 4 (KRT4)

Recombinant Human Keratin, type II cytoskeletal 4 (KRT4) — Structural component of intermediate filaments in mucosal stratified squamous epithelia. Purity >90%.

SKU: BCRECP-001041 Category:
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Product Specifications

Product SkuBCRECP-001041
Product DescriptionRecombinant Human Keratin, type II cytoskeletal 4 (KRT4) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-534aa. [Accession | P19013].
Uniprot No.P19013
Gene NamesKRT4
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-534aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight73.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Structural component of intermediate filaments in mucosal stratified squamous epithelia. Forms heteropolymers with the type I keratin KRT13, assembling into keratin intermediate filament networks that provides structural integrity and mechanical stability of the mucosal epithelium.

Biological Context

Tissue Specificity: Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer
Disease Association: White sponge nevus 1 (WSN1) : A rare disorder characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Keratin, type II cytoskeletal 4 (KRT4) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-534aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 73.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Structural component of intermediate filaments in mucosal stratified squamous epithelia. Forms heteropolymers with the type I keratin KRT13, assembling into keratin intermediate filament networks that provides structural integrity and mechanical stability of the mucosal epithelium

Disease Association

White sponge nevus 1 (WSN1) : A rare disorder characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer

Subunit

Heterodimers composed of one type I and one type II keratins; forms parallel coiled-coil heterodimers (By similarity). Heterodimers associate in an antiparallel manner to form heterotetramers, which further assemble into higher-order keratin intermediate filaments (By similarity)

Gene: KRT4  |  Organism: Homo sapiens  |  Synonyms: Cytokeratin-4; Keratin-4; Type-II keratin Kb4
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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