Recombinant Human L-lactate dehydrogenase A chain (LDHA), Homo sapiens (Human), Yeast, N-terminal 6xHis-tagged, 38.6kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human L-lactate dehydrogenase A chain (LDHA)

Recombinant Human L-lactate dehydrogenase A chain (LDHA) — recombinant protein from Homo sapiens (Human), expressed in Yeast. Purity >85%.

SKU: BCRECP-001059 Categories: ,
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Product Specifications

Product SkuBCRECP-001059
Product DescriptionRecombinant Human L-lactate dehydrogenase A chain (LDHA) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 2-332aa. [Accession | P00338].
Uniprot No.P00338
Gene NamesLDHA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region2-332aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight38.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Predominantly expressed in anaerobic tissues such as skeletal muscle and liver
Disease Association: Glycogen storage disease 11 (GSD11) : A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1

Product Specifications

Recombinant Human L-lactate dehydrogenase A chain (LDHA) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 2-332aa, with N-terminal 6xHis-tagged tag, molecular weight 38.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)

Subcellular Location

Cytoplasm

Disease Association

Glycogen storage disease 11 (GSD11) : A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Predominantly expressed in anaerobic tissues such as skeletal muscle and liver

Subunit

Homotetramer (PubMed:11276087). Interacts with PTEN upstream reading frame protein MP31 (PubMed:33406399). Interacts with folliculin FLCN; the interaction is direct and inhibits enzymatic activity (PubMed:34381247)

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1

Gene: LDHA  |  Organism: Homo sapiens  |  Synonyms: Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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