Recombinant Human Large neutral amino acids transporter small subunit 1 (SLC7A5), partial

Function

(Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis

Subcellular Location

Apical cell membrane (Multi-pass membrane protein); Cell membrane (Multi-pass membrane protein); Lysosome membrane (Multi-pass membrane protein)

Tissue Specificity

Detected in placenta, in the syncytiotrophoblast layer (at protein level) (PubMed:11389679). Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2

Subunit

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc (PubMed:10049700, PubMed:10574970, PubMed:11389679, PubMed:11557028, PubMed:11564694, PubMed:12117417, PubMed:12225859, PubMed:15769744, PubMed:25998567, PubMed:30867591, PubMed:9751058). Interacts with LAPTM4B; this recruits the heterodimer formed by SLC3A2/4F2hc and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567)

• Mastroberardino L. et al. Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. — Nature (1998)
• Prasad P.D. et al. Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function. — Biochem. Biophys. Res. Commun. (1999)
• Tsurudome M. et al. Primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16. — J. Immunol. (1999)
• Yanagida O. et al. Human L-type amino acid transporter 1 (LAT1): characterization of function and expression in tumor cell lines. — Biochim. Biophys. Acta (2001)
• Minato N. et al. Human 4F2 light chain: amino acid transporter. (SEP-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Gaugitsch H.W. et al. A novel transiently expressed, integral membrane protein linked to cell activation. Molecular cloning via the rapid degradation signal AUUUA. — J. Biol. Chem. (1992)
• Rossier G. et al. LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine. — J. Biol. Chem. (1999)