Recombinant Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 47.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM)

Recombinant Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM) — Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acet… Purity >…

SKU: BCRECP-000011 Categories: ,
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Product Specifications

Product SkuBCRECP-000011
Product DescriptionRecombinant Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 26-421aa. [Accession | P11310].
Uniprot No.P11310
Gene NamesACADM
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region26-421aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight47.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). ETF is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Biological Context

Subcellular Location: Mitochondrion matrix
Tissue Specificity: Expressed ubiquitously with highest levels in heart and muscle
Disease Association: Acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) : An inborn error of mitochondrial fatty acid beta-oxidation which causes fasting hypoglycemia, hepatic dysfunction and encephalopathy, often resulting in death in infancy. [The disease is caused by variants affecting the gene represented in this entry]
Pathway: Lipid metabolism; mitochondrial fatty acid beta-oxidation

Product Specifications

Recombinant Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 26-421aa, with N-terminal 6xHis-tagged tag, molecular weight 47.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed:2251268). ETF is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:15159392, PubMed:25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase has preference for fatty acyl-CoAs with saturated 6 to 12 carbons long primary chains, making it but can also catalyze longer chains such as C14 and C16 (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175)

Subcellular Location

Mitochondrion matrix

Disease Association

Acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) : An inborn error of mitochondrial fatty acid beta-oxidation which causes fasting hypoglycemia, hepatic dysfunction and encephalopathy, often resulting in death in infancy. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed ubiquitously with highest levels in heart and muscle

Subunit

Homotetramer (PubMed:8823176, Ref.24). Interacts with the heterodimeric electron transfer flavoprotein ETF

Pathway

Lipid metabolism; mitochondrial fatty acid beta-oxidation

Gene: ACADM  |  Organism: Homo sapiens  |  Synonyms: Medium chain acyl-CoA dehydrogenase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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