Recombinant Human metapneumovirus Fusion glycoprotein F0 (F) Protein, Human metapneumovirus (strain CAN97-83) (HMPV), in vitro E.coli expression system, N-terminal 6xHis-SUMO-tagged, 72.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human metapneumovirus Fusion glycoprotein F0 (F) Protein

Recombinant Human metapneumovirus Fusion glycoprotein F0 (F) Protein — Major determinant of the species specificity of RSV infection. Purity >85%.

SKU: BCRECP-00435TM Categories: ,
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Product Specifications

Uniprot No.Q6WB98
Gene NamesF
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region19-539aa
SpeciesHuman metapneumovirus (strain CAN97-83) (HMPV)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight72.4kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Major determinant of the species specificity of RSV infection. The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate.

Biological Context

Subcellular Location: Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass membrane protein)

Product Specifications

Recombinant Human metapneumovirus Fusion glycoprotein F0 (F) Protein is a recombinant protein from Human metapneumovirus (strain CAN97-83) (HMPV), expressed in in vitro E.coli expression system, covering amino acids 19-539aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 72.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Major determinant of the species specificity of RSV infection (By similarity). The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate (PubMed:22238303)

Subcellular Location

Virion membrane (Single-pass type I membrane protein); Host cell membrane (Single-pass membrane protein)

Subunit

Homotrimer. Heterodimer with fusion protein F1; disulfide-linked. As a heterodimer with F1, interacts with host heparan sulfate (PubMed:22238303). As a heterodimer with F2, interacts with host integrin ITGAV/ITGB1 (PubMed:19164533). Part of a complex composed of F1, F2 and G glycoproteins (By similarity)

Gene: F  |  Organism: Human metapneumovirus (strain CAN97-83)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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