Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-Myc-tagged, 56.9 kDa, purity >95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
,

Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial (Active)

Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial (Active) — Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Purity >95%.

SKU: BCACP-000063 Categories: ,
Online Inquiry →
Product SKUBCACP-000063
Product DescriptionRecombinant Human Myeloid cell surface antigen CD33 (CD33) protein is expressed from Mammalian cell with C-terminal hFc1-Myc-tagged. It contains 18-259aa. [Accession | P20138].
Uniprot No.P20138
Gene NamesCD33
PurityGreater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region18-259aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-Myc-tagged
Molecular weight56.9 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesCD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33

Function

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK.

Biological Context

Subcellular Location: Peroxisome
Tissue Specificity: Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells

Product Specifications

Recombinant Human Myeloid cell surface antigen CD33 (CD33), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 18-259aa, with C-terminal hFc1-Myc-tagged tag, molecular weight 56.9 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:11320212, PubMed:15597323). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:10887109, PubMed:28325905). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10206955, PubMed:10556798, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323)

Subcellular Location

Peroxisome

Tissue Specificity

Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells

Subunit

Homodimer; disulfide-linked (PubMed:10887109). Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation (PubMed:10206955, PubMed:10556798). Interacts with C1QA (via C-terminus); this interaction activates CD33 inhibitory motifs (PubMed:28325905)

Gene: CD33  |  Organism: Homo sapiens  |  Synonyms: Sialic acid-binding Ig-like lectin 3; gp67
Key Publications

Explore closely related product collections, technology platforms, and custom production resources.

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

Shopping Cart
Scroll to Top