| Product SKU | BCACP-000063 |
| Product Description | Recombinant Human Myeloid cell surface antigen CD33 (CD33) protein is expressed from Mammalian cell with C-terminal hFc1-Myc-tagged. It contains 18-259aa. [Accession | P20138]. |
| Uniprot No. | P20138 |
| Gene Names | CD33 |
| Purity | Greater than 95% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC. |
| Expression System | Mammalian cell |
| Expression Region | 18-259aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal hFc1-Myc-tagged |
| Molecular weight | 56.9 kDa |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized CD152 at 2 μg/ml can bind Anti-CD152 rabbit monoclonal antibody, the EC50 of human CD152 protein is 27.14-34.82 ng/ml. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD antigen CD33 |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
