| Product SKU | BCACP-000848 |
| Product Description | Recombinant Human NAD-dependent malic enzyme, mitochondrial protein (ME2) protein is expressed from E.Coli with C-terminal 6xHis-tagged. It contains 19-584aa. [Accession | P23368]. |
| Uniprot No. | P23368 |
| Gene Names | ME2 |
| Purity | >95% as determined by SDS-PAGE. |
| Expression System | E.Coli |
| Expression Region | 19-584aa |
| Species | Homo sapiens (Human) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 64.4 kDa |
| Activity | Fully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 3000 U/mg. |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Alternative Names | Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD(+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD(+) dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase |
SDS-PAGE: Single band at expected molecular weight confirming purity.
ELISA: Suitable as coating antigen or detection standard.
Western Blot: Compatible with standard Western Blot protocols.
Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.
Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.
Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.
Shelf Life: 12 months from date of receipt when stored as recommended.
Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.
