Recombinant Human parechovirus 1 Genome polyprotein, partial, Human parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 39.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human parechovirus 1 Genome polyprotein, partial

Recombinant Human parechovirus 1 Genome polyprotein, partial — Replicates the viral genomic RNA on the surface of intracellular membranes. Purity >85%.

SKU: BCRECP-003749 Categories: ,
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Product Specifications

Product SkuBCRECP-003749
Product DescriptionRecombinant Human parechovirus 1 Genome polyprotein, partial Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-289aa. [Accession | Q66578].
Uniprot No.Q66578
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-289aa
SpeciesHuman parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight39.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Replicates the viral genomic RNA on the surface of intracellular membranes. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form.

Biological Context

Subcellular Location: Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Product Specifications

Recombinant Human parechovirus 1 Genome polyprotein, partial is a recombinant protein from Human parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22), expressed in E.coli, covering amino acids 1-289aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 39.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Replicates the viral genomic RNA on the surface of intracellular membranes. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated

Subcellular Location

Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Subunit

Interacts with Viral protein genome-linked

Organism: Human parechovirus 1 (strain Harris)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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