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Recombinant Human parechovirus 1 Genome polyprotein

Recombinant Human parechovirus 1 Genome polyprotein — Replicates the viral genomic RNA on the surface of intracellular membranes. Purity >90%.

SKU: BCRECP-003831 Categories: ,

Product Specifications

Product SkuBCRECP-003831
Product DescriptionRecombinant Human parechovirus 1 Genome polyprotein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 1-289aa. [Accession | Q66578].
Uniprot No.Q66578
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-289aa
SpeciesHuman parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22)
Tag InfoC-terminal 6xHis-tagged
Molecular weight33.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Replicates the viral genomic RNA on the surface of intracellular membranes. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form.

Biological Context

Subcellular Location: Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Product Specifications

Recombinant Human parechovirus 1 Genome polyprotein is a recombinant protein from Human parechovirus 1 (strain Harris) (HPeV-1) (Echovirus 22), expressed in Yeast, covering amino acids 1-289aa, with C-terminal 6xHis-tagged tag, molecular weight 33.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Replicates the viral genomic RNA on the surface of intracellular membranes. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated

Subcellular Location

Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Subunit

Interacts with Viral protein genome-linked

Organism: Human parechovirus 1 (strain Harris)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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