Recombinant Human Probable DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C), Homo sapiens (Human), E.coli, N-terminal GST-tagged, 49.8kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Probable DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C)

Recombinant Human Probable DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C) — DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Purity >90%.

SKU: BCRECP-000108 Categories: ,
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Product Specifications

Product SkuBCRECP-000108
Product DescriptionRecombinant Human Probable DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 1-190aa. [Accession | Q9NRW3].
Uniprot No.Q9NRW3
Gene NamesAPOBEC3C
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-190aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight49.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells.

Biological Context

Subcellular Location: Nucleus; Cytoplasm
Tissue Specificity: Expressed in spleen, testes, peripherical blood lymphocytes, heart, thymus, prostate and ovary

Product Specifications

Recombinant Human Probable DNA dC->dU-editing enzyme APOBEC-3C (APOBEC3C) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-190aa, with N-terminal GST-tagged tag, molecular weight 49.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV), herpes simplex virus 1 (HHV-1) and Epstein-Barr virus (EBV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation

Subcellular Location

Nucleus; Cytoplasm

Tissue Specificity

Expressed in spleen, testes, peripherical blood lymphocytes, heart, thymus, prostate and ovary

Subunit

(Microbial infection) Interacts with HIV-1 Vif

Gene: APOBEC3C  |  Organism: Homo sapiens  |  Synonyms: APOBEC1-like; Phorbolin I
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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