Recombinant Human Protein max (MAX)

Function

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements

Subcellular Location

Nucleus; Cell projection, dendrite

Disease Association

Pheochromocytoma (PCC) : A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. [Disease susceptibility is associated with variants affecting the gene represented in this entry] | Polydactyly-macrocephaly syndrome (PDMCS) : An autosomal dominant syndrome characterized by progressive macrocephaly and post-axial polydactyly, a condition defined by the occurrence of supernumerary digits affecting the fifth finger and/or toe. Additional variable features include ocular anomalies, global developmental delay and autistic traits. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle

Subunit

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BACC1, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity

• Blackwood E.M. et al. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. — Science (1991)
• Vaestrik I. et al. Alternative mRNA forms and open reading frames of the max gene. — Oncogene (1993)
• Maekelae T.P. et al. Alternative forms of Max as enhancers or suppressors of Myc-ras cotransformation. — Science (1992)
• Ota T. et al. Complete sequencing and characterization of 21,243 full-length human cDNAs. — Nat. Genet. (2004)
• Heilig R. et al. The DNA sequence and analysis of human chromosome 14. — Nature (2003)
• Mural R.J. et al. PubMed: CI-5GBDQ6B103N1E (JUL-)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Ogawa H. et al. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells. — Science (2002)