Recombinant Human Protein mono-ADP-ribosyltransferase PARP14 (PARP14), partial, Homo sapiens (Human), Baculovirus, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 26.4kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human Protein mono-ADP-ribosyltransferase PARP14 (PARP14), partial

Recombinant Human Protein mono-ADP-ribosyltransferase PARP14 (PARP14), partial — ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins. Purity >85%.

SKU: BCRECP-001333 Categories: ,
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Product Specifications

Product SkuBCRECP-001333
Product DescriptionRecombinant Human Protein mono-ADP-ribosyltransferase PARP14 (PARP14) Protein is expressed from Baculovirus with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1605-1801aa. [Accession | Q460N5].
Uniprot No.Q460N5
Gene NamesPARP14
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemBaculovirus
Expression Region1605-1801aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight26.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation.

Biological Context

Subcellular Location: Nucleus; Cytoplasm
Tissue Specificity: Expressed in macrophages

Product Specifications

Recombinant Human Protein mono-ADP-ribosyltransferase PARP14 (PARP14), partial is a recombinant protein from Homo sapiens (Human), expressed in Baculovirus, covering amino acids 1605-1801aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 26.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed:16061477, PubMed:18851833, PubMed:25043379, PubMed:27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed:27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed:29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed:27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed:27796300). Mono-ADP-ribosylates PARP9 (PubMed:27796300)

Subcellular Location

Nucleus; Cytoplasm

Tissue Specificity

Expressed in macrophages

Subunit

Interacts with STAT6 (By similarity). Interacts with PARP10 (PubMed:23473667). Interacts with PARP9 in IFNG-stimulated macrophages; the interaction prevents PARP14-mediated STAT1 and STAT6 ADP-riboslylation (PubMed:27796300)

Gene: PARP14  |  Organism: Homo sapiens  |  Synonyms: ADP-ribosyltransferase diphtheria toxin-like 8; B aggressive lymphoma protein 2; Poly [ADP-ribose] polymerase 14
Key Publications

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