Recombinant Human Ras GTPase-activating protein 1 (RASA1), partial, Homo sapiens (Human), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 45.6kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Ras GTPase-activating protein 1 (RASA1), partial

Recombinant Human Ras GTPase-activating protein 1 (RASA1), partial — recombinant protein from Homo sapiens (Human), expressed in E.coli. Purity >85%.

SKU: BCRECP-001520 Category:
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Product Specifications

Product SkuBCRECP-001520
Product DescriptionRecombinant Human Ras GTPase-activating protein 1 (RASA1) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 714-1047aa. [Accession | P20936].
Uniprot No.P20936
Gene NamesRASA1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region714-1047aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight45.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level)
Disease Association: [Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas] | Capillary malformation-arteriovenous malformation 1 (CMAVM1) : A disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. CMAVM1 inheritance is autosomal dominant. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Ras GTPase-activating protein 1 (RASA1), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 714-1047aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 45.6kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

GTPase-activating protein (GAP) that stimulates the intrinsic GTPase activity of Ras proteins, such as NRAS, facilitating their transition from the active GTP-bound state to the inactive GDP-bound state, thereby terminating Ras signaling

Subcellular Location

Cytoplasm

Disease Association

[Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas] | Capillary malformation-arteriovenous malformation 1 (CMAVM1) : A disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome. CMAVM1 inheritance is autosomal dominant. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level)

Subunit

Interacts with SQSTM1. Interacts with SPSB1; the interaction does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1. Interacts with tyrosine-phosphorylated EPHB4 (PubMed:30578106)

Gene: RASA1  |  Organism: Homo sapiens  |  Synonyms: Ras p21 protein activator; p120GAP
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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