Recombinant Human Ras-related protein Rab-33A (RAB33A), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 28.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Human Ras-related protein Rab-33A (RAB33A)

Recombinant Human Ras-related protein Rab-33A (RAB33A) — The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Purity >85%.

SKU: BCRECP-001504 Category:
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Product Specifications

Product SkuBCRECP-001504
Product DescriptionRecombinant Human Ras-related protein Rab-33A (RAB33A) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1-237aa. [Accession | Q14088].
Uniprot No.Q14088
Gene NamesRAB33A
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region1-237aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight28.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Modulates autophagosome formation through interaction with ATG16L1.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor)
Tissue Specificity: Expressed only in lymphoid cell lines

Product Specifications

Recombinant Human Ras-related protein Rab-33A (RAB33A) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 1-237aa, with C-terminal 10xHis-tagged tag, molecular weight 28.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (By similarity). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Modulates autophagosome formation through interaction with ATG16L1 (By similarity)

Subcellular Location

Cell membrane (Lipid-anchor)

Tissue Specificity

Expressed only in lymphoid cell lines

Subunit

Interacts with ATG16L1; the interaction is important for autophagosome formation

Gene: RAB33A  |  Organism: Homo sapiens  |  Synonyms: Small GTP-binding protein S10

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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