Recombinant Human Retinoic acid-induced protein 3 (GPRC5A), partial, Homo sapiens (Human), E.coli, N-terminal GST-tagged, 37.0kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Retinoic acid-induced protein 3 (GPRC5A), partial

Recombinant Human Retinoic acid-induced protein 3 (GPRC5A), partial — Orphan receptor. Purity >90%.

SKU: BCRECP-000781 Category:
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Product Specifications

Product SkuBCRECP-000781
Product DescriptionRecombinant Human Retinoic acid-induced protein 3 (GPRC5A) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 269-357aa. [Accession | Q8NFJ5].
Uniprot No.Q8NFJ5
Gene NamesGPRC5A
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region269-357aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal GST-tagged
Molecular weight37.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G protein signaling pathways.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed at high level in fetal and adult lung tissues but repressed in most human lung cancers. Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal cord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart

Product Specifications

Recombinant Human Retinoic acid-induced protein 3 (GPRC5A), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 269-357aa, with N-terminal GST-tagged tag, molecular weight 37.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor (PubMed:18000218)

Subcellular Location

Cell membrane (Multi-pass membrane protein); Cytoplasmic vesicle membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed at high level in fetal and adult lung tissues but repressed in most human lung cancers (PubMed:18000218, PubMed:9857033). Constitutively expressed in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal cord at low to moderate levels. Not detectable in fetal heart, brain, and liver and adult heart, brain, liver, skeletal muscle, pancreas, spleen, thymus, and peripheral leukocytes. According to PubMed:10783259, expressed at low but detectable level in pancreas and heart

Subunit

Interacts (via its transmembrane domain) with EGFR

Gene: GPRC5A  |  Organism: Homo sapiens  |  Synonyms: G protein-coupled receptor family C group 5 member A; Phorbol ester induced gene 1; Retinoic acid-induced gene 1 protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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