Recombinant Human Retinol-binding protein 4 (RBP4) (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-tagged, 50.0 kDa, purity >90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.

Recombinant Human Retinol-binding protein 4 (RBP4) (Active)

Recombinant Human Retinol-binding protein 4 (RBP4) (Active) — Retinol-binding protein that mediates retinol transport in blood plasma. Purity >90%.

SKU: BCACP-000085 Category:
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Product SKUBCACP-000085
Product DescriptionRecombinant Human Retinol-binding protein 4 (RBP4) protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 19-201aa. [Accession | P02753].
Uniprot No.P02753
Gene NamesRBP4
PurityGreater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region19-201aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-tagged
Molecular weight50.0 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized TNFRSF14 at 5 μg/ml can bind TNFSF14, the EC50 is 45.44-53.29 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(Plasma retinol-binding protein)(PRBP)(RBP)

Function

Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Detected in blood plasma and in urine (at protein level)
Disease Association: Retinal dystrophy, iris coloboma, and comedogenic acne syndrome (RDCCAS) : A disease characterized by retinal degeneration, ocular colobomas involving both the anterior and posterior segment, impaired night vision and loss of visual acuity. Additional characteristic features include developmental abnormalities and severe acne. [The disease is caused by variants affecting the gene represented in this entry. Loss of functional RBP4 protein results in serum retinol deficiency. Lack of normal levels of retinol impairs the visual cycle leading to night blindness at early stages; prolonged deficiency may lead to retinal degeneration. Additionally, retinol deficiency may result in dry skin, increased susceptibility to infection and acne ] | Microphthalmia/Coloboma 10 (MCOPCB10) : A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, and other abnormalities may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Retinol-binding protein 4 (RBP4) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 19-201aa, with C-terminal hFc1-tagged tag, molecular weight 50.0 kDa, purity Greater than 90% as determined by SDS-PAGE.Greater than 90% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Retinol-binding protein that mediates retinol transport in blood plasma (PubMed:5541771). Delivers retinol from the liver stores to the peripheral tissues (Probable). Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane (PubMed:22665496)

Subcellular Location

Secreted

Disease Association

Retinal dystrophy, iris coloboma, and comedogenic acne syndrome (RDCCAS) : A disease characterized by retinal degeneration, ocular colobomas involving both the anterior and posterior segment, impaired night vision and loss of visual acuity. Additional characteristic features include developmental abnormalities and severe acne. [The disease is caused by variants affecting the gene represented in this entry. Loss of functional RBP4 protein results in serum retinol deficiency. Lack of normal levels of retinol impairs the visual cycle leading to night blindness at early stages; prolonged deficiency may lead to retinal degeneration. Additionally, retinol deficiency may result in dry skin, increased susceptibility to infection and acne (PubMed:23189188)] | Microphthalmia/Coloboma 10 (MCOPCB10) : A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, and other abnormalities may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Detected in blood plasma and in urine (at protein level)

Subunit

Interacts with TTR (PubMed:10052934, PubMed:19021760, PubMed:5541771, PubMed:7754382). Interaction with TTR prevents its loss by filtration through the kidney glomeruli (Probable). Interacts with STRA6 (PubMed:22665496, PubMed:25910211)

Gene: RBP4  |  Organism: Homo sapiens  |  Synonyms: Plasma retinol-binding protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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