Recombinant Human rhinovirus 1B Genome polyprotein, partial, Human rhinovirus 1B (HRV-1B), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 43.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human rhinovirus 1B Genome polyprotein, partial

Recombinant Human rhinovirus 1B Genome polyprotein, partial — Major viral protease that mediates proteolytic processing of the polyprotein. Purity >85%.

SKU: BCRECP-003588 Categories: ,
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Product Specifications

Product SkuBCRECP-003588
Product DescriptionRecombinant Human rhinovirus 1B Genome polyprotein, partial Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-332aa. [Accession | P12916].
Uniprot No.P12916
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-332aa
SpeciesHuman rhinovirus 1B (HRV-1B)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight43.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Major viral protease that mediates proteolytic processing of the polyprotein. Cleaves host EIF5B, contributing to host translation shutoff. Also cleaves host PABPC1, contributing to host translation shutoff.

Biological Context

Subcellular Location: Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Product Specifications

Recombinant Human rhinovirus 1B Genome polyprotein, partial is a recombinant protein from Human rhinovirus 1B (HRV-1B), expressed in E.coli, covering amino acids 2-332aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 43.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Major viral protease that mediates proteolytic processing of the polyprotein (By similarity). Cleaves host EIF5B, contributing to host translation shutoff (By similarity). Also cleaves host PABPC1, contributing to host translation shutoff (By similarity). Cleaves host NLRP1, triggers host N-glycine-mediated degradation of the autoinhibitory NLRP1 N-terminal fragment (By similarity)

Subcellular Location

Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Subunit

Interacts with Viral protein genome-linked and with protein 3CD

Organism: Human rhinovirus 1B

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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