Recombinant Human rhinovirus 1B Genome polyprotein, partial, Human rhinovirus 1B (HRV-1B), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 43.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Human rhinovirus 1B Genome polyprotein, partial

Recombinant Human rhinovirus 1B Genome polyprotein, partial — Major viral protease that mediates proteolytic processing of the polyprotein. Purity >85%.

SKU: BCRECP-003588 Categories: ,
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Product Specifications

Product SkuBCRECP-003588
Product DescriptionRecombinant Human rhinovirus 1B Genome polyprotein, partial Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 2-332aa. [Accession | P12916].
Uniprot No.P12916
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-332aa
SpeciesHuman rhinovirus 1B (HRV-1B)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight43.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Major viral protease that mediates proteolytic processing of the polyprotein. Cleaves host EIF5B, contributing to host translation shutoff. Also cleaves host PABPC1, contributing to host translation shutoff.

Biological Context

Subcellular Location: Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Product Specifications

Recombinant Human rhinovirus 1B Genome polyprotein, partial is a recombinant protein from Human rhinovirus 1B (HRV-1B), expressed in E.coli, covering amino acids 2-332aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 43.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Major viral protease that mediates proteolytic processing of the polyprotein (By similarity). Cleaves host EIF5B, contributing to host translation shutoff (By similarity). Also cleaves host PABPC1, contributing to host translation shutoff (By similarity). Cleaves host NLRP1, triggers host N-glycine-mediated degradation of the autoinhibitory NLRP1 N-terminal fragment (By similarity)

Subcellular Location

Host cytoplasmic vesicle membrane (Peripheral membrane protein)

Subunit

Interacts with Viral protein genome-linked and with protein 3CD

Organism: Human rhinovirus 1B

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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