Recombinant Human RNA demethylase ALKBH5 (ALKBH5), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 48.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human RNA demethylase ALKBH5 (ALKBH5)

Recombinant Human RNA demethylase ALKBH5 (ALKBH5) — Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Purity >90%.

SKU: BCRECP-000063 Category:
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Product Specifications

Product SkuBCRECP-000063
Product DescriptionRecombinant Human RNA demethylase ALKBH5 (ALKBH5) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-394aa. [Accession | Q6P6C2].
Uniprot No.Q6P6C2
Gene NamesALKBH5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-394aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight48.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing, translation and export.

Biological Context

Subcellular Location: Nucleus speckle; Nucleus, nucleoplasm
Tissue Specificity: Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary

Product Specifications

Recombinant Human RNA demethylase ALKBH5 (ALKBH5) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-394aa, with N-terminal 6xHis-tagged tag, molecular weight 48.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Dioxygenase that specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178, PubMed:34048572, PubMed:36944332, PubMed:37257451, PubMed:37369679, PubMed:40793791). Demethylates RNA by oxidative demethylation, which requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing, translation and export (PubMed:23177736, PubMed:34048572, PubMed:36944332, PubMed:37257451). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells (By similarity). Involved in paraspeckle assembly, a nuclear membraneless organelle, by undergoing liquid-liquid phase separation (PubMed:37369679, PubMed:37474102). Paraspeckle assembly is coupled with m6A demethylation of RNAs, such as NEAT1 non-coding RNA (PubMed:37474102). Also acts as a negative regulator of T-cell development: inhibits gamma-delta T-cell proliferation via demethylation of JAG1 and NOTCH2 transcripts (By similarity). Inhibits regulatory T-cell (Treg) recruitment by mediating demethylation and destabilization of CCL28 mRNAs (By similarity)

Subcellular Location

Nucleus speckle; Nucleus, nucleoplasm

Tissue Specificity

Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary

Subunit

Monomer (PubMed:24778178). Interacts with RBM33; promoting desumoylation by SENP1 and recruitment to N(6)-methyladenosine-containing mRNAs (PubMed:37257451). Interacts (when acetylated by KAT8) with PSPC1; interaction facilitates recognition of N(6)-methyladenosine (m6A) mRNA (PubMed:37369679). Interacts with DDX54; this interaction down-regulates the interferon antiviral response (PubMed:40793791)

Gene: ALKBH5  |  Organism: Homo sapiens  |  Synonyms: Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
Key Publications

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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