Recombinant Human Semaphorin-7A (SEMA7A), Homo sapiens (Human), Yeast, N-terminal 6xHis-tagged, 70.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Semaphorin-7A (SEMA7A)

Recombinant Human Semaphorin-7A (SEMA7A) — Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Purity >90%.

SKU: BCRECP-001605 Category:
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Product Specifications

Product SkuBCRECP-001605
Product DescriptionRecombinant Human Semaphorin-7A (SEMA7A) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 45-648aa. [Accession | O75326].
Uniprot No.O75326
Gene NamesSEMA7A
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region45-648aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight70.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor, GPI-anchor)
Tissue Specificity: Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal cord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney
Disease Association: Cholestasis, progressive familial intrahepatic, 11 (PFIC11) : An autosomal recessive form of progressive cholestasis, a disorder characterized by early onset of cholestasis that progresses to hepatic fibrosis, cirrhosis, and end-stage liver disease. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Semaphorin-7A (SEMA7A) is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 45-648aa, with N-terminal 6xHis-tagged tag, molecular weight 70.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of pro-inflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes

Subcellular Location

Cell membrane (Lipid-anchor, GPI-anchor)

Disease Association

Cholestasis, progressive familial intrahepatic, 11 (PFIC11) : An autosomal recessive form of progressive cholestasis, a disorder characterized by early onset of cholestasis that progresses to hepatic fibrosis, cirrhosis, and end-stage liver disease. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Detected in skin keratinocytes and on endothelial cells from skin blood vessels (at protein level). Expressed in fibroblasts, keratinocytes, melanocytes, placenta, testis, ovary, spleen, brain, spinal cord, lung, heart, adrenal gland, lymph nodes, thymus, intestine and kidney

Subunit

Interacts with ITGA1 and ITGB1 (Probable). Interacts with PLXNC1

Gene: SEMA7A  |  Organism: Homo sapiens  |  Synonyms: CDw108; JMH blood group antigen; John-Milton-Hargen human blood group Ag; Semaphorin-K1; Semaphorin-L
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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