Recombinant Human Signal-regulatory protein beta-1 (SIRPB1), partial (Active), Homo sapiens (Human), Mammalian cell, C-terminal 10xHis-tagged, 38.6 kDa, purity >95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.

Recombinant Human Signal-regulatory protein beta-1 (SIRPB1), partial (Active)

Recombinant Human Signal-regulatory protein beta-1 (SIRPB1), partial (Active) — Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Purity >95%.

SKU: BCACP-000611 Category:
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Product SKUBCACP-000611
Product DescriptionRecombinant Human Signal-regulatory protein beta-1 (SIRPB1) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 30-371aa. [Accession | O00241].
Uniprot No.O00241
Gene NamesSIRPB1
PurityGreater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.
Expression SystemMammalian cell
Expression Region30-371aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 10xHis-tagged
Molecular weight38.6 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human C5 at 2 μg/mL can bind Anti-C5 recombinant antibody . The EC50 is 1.867-2.192 ng/mL.;C5 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Human C5 with an affinity constant of 0.285 nM as detected by MetaSPR Assay (WeSPRTM 200).
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesSignal-regulatory protein beta-1; SIRP-beta-1; CD172 antigen-like family member B; CD antigen CD172b

Function

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Also participates in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Detected in monocytes and dendritic cells

Product Specifications

Recombinant Human Signal-regulatory protein beta-1 (SIRPB1), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 30-371aa, with C-terminal 10xHis-tagged tag, molecular weight 38.6 kDa, purity Greater than 95% as determined by SDS-PAGE.Greater than 95% as determined by SEC-HPLC.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Also participates in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP (PubMed:10604985)

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Detected in monocytes and dendritic cells

Subunit

Homodimer; disulfide-linked. Interacts with TYROBP. This interaction results in the recruitment of SYK

Gene: SIRPB1  |  Organism: Homo sapiens  |  Synonyms: CD172 antigen-like family member B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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