Recombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2), Biotinylated, Homo sapiens (Human), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 101.2kDa, purity >85% as determined by SDS-PAGE.
,

Recombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2), Biotinylated

Recombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2), Biotinylated — Plays a role in pre-mRNA splicing and 3′-end processing. Purity >85%.

SKU: BCRECP-001880 Categories: ,
Online Inquiry →

Product Specifications

Product SkuBCRECP-001880
Product DescriptionRecombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 2-475aa. [Accession | P26368].
Uniprot No.P26368
Gene NamesU2AF2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-475aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight101.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle.

Biological Context

Subcellular Location: Nucleus
Disease Association: Developmental delay, dysmorphic facies, and brain anomalies (DEVDFB) : An autosomal dominant disorder characterized by global developmental delay with intellectual disability and speech delay, epilepsy, hypotonia, short stature, microcephaly, intermittent exotropia, and non-specific facial dysmorphism. Brain imaging shows a thin corpus callosum and/or delayed myelination. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Splicing factor U2AF 65 kDa subunit (U2AF2), Biotinylated is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 2-475aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 101.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186). By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186)

Subcellular Location

Nucleus

Disease Association

Developmental delay, dysmorphic facies, and brain anomalies (DEVDFB) : An autosomal dominant disorder characterized by global developmental delay with intellectual disability and speech delay, epilepsy, hypotonia, short stature, microcephaly, intermittent exotropia, and non-specific facial dysmorphism. Brain imaging shows a thin corpus callosum and/or delayed myelination. [The disease is caused by variants affecting the gene represented in this entry]

Subunit

Interacts with U2AF1L4 (By similarity). Heterodimer with U2AF1 (PubMed:11551507). Binds unphosphorylated SF1 (PubMed:10449420, PubMed:12718882). Interacts with SCAF11 and SNW1 (PubMed:21460037, PubMed:9447963). Interacts with ZRSR2/U2AF1-RS2. Interacts with RBM17 (PubMed:17589525). Interacts with PRPF19; the interaction is direct. Interacts with POLR2A (via the C-terminal domain); recruits PRPF19 and the Prp19 complex to the pre-mRNA (PubMed:21536736). Interacts with KHDC4 (Isoform 2) (PubMed:19641227). Interacts with ZRSR2 (PubMed:9237760). Interacts with the SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A (PubMed:27720643). Interacts (via N-terminus) with CPSF7 (via C-terminus); this interaction stimulates pre-mRNA 3'-end processing by promoting the recruitment of the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186). Interacts with ARGLU1; interaction may be involved in ARGLU1-mediated modulation of alternative splicing (PubMed:30698747)

Gene: U2AF2  |  Organism: Homo sapiens  |  Synonyms: U2 auxiliary factor 65 kDa subunit; U2 snRNP auxiliary factor large subunit
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top