Recombinant Human Telomerase-binding protein EST1A (SMG6), partial, Homo sapiens (Human), Yeast, C-terminal 6xHis-tagged, 21.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Telomerase-binding protein EST1A (SMG6), partial

Recombinant Human Telomerase-binding protein EST1A (SMG6), partial — Plays a role in nonsense-mediated mRNA decay. Purity >90%.

SKU: BCRECP-001667 Category:
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Product Specifications

Product SkuBCRECP-001667
Product DescriptionRecombinant Human Telomerase-binding protein EST1A (SMG6) Protein is expressed from Yeast with C-terminal 6xHis-tagged. It contains 1239-1419aa. [Accession | Q86US8].
Uniprot No.Q86US8
Gene NamesSMG6
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1239-1419aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight21.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA.

Biological Context

Subcellular Location: Nucleus, nucleolus; Chromosome, telomere; Cytoplasm, cytosol
Tissue Specificity: Ubiquitous

Product Specifications

Recombinant Human Telomerase-binding protein EST1A (SMG6), partial is a recombinant protein from Homo sapiens (Human), expressed in Yeast, covering amino acids 1239-1419aa, with C-terminal 6xHis-tagged tag, molecular weight 21.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Plays a role in nonsense-mediated mRNA decay (PubMed:17053788, PubMed:18974281, PubMed:19060897, PubMed:20930030). Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation (PubMed:17053788, PubMed:18974281, PubMed:19060897, PubMed:20930030). Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA (PubMed:17053788, PubMed:18974281, PubMed:19060897, PubMed:20930030)

Subcellular Location

Nucleus, nucleolus; Chromosome, telomere; Cytoplasm, cytosol

Tissue Specificity

Ubiquitous

Subunit

May form homooligomers (PubMed:20930030). Associated component of the telomerase holoenzyme complex (PubMed:19179534). Interacts with TERT, independently of the telomerase RNA (PubMed:12676087, PubMed:12699629). Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits (PubMed:12554878, PubMed:19060897, PubMed:20930030). Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay (PubMed:20930030). Interacts with DHX34; the interaction is RNA-independent (PubMed:25220460)

Gene: SMG6  |  Organism: Homo sapiens  |  Synonyms: Ever shorter telomeres 1A; Nonsense mediated mRNA decay factor SMG6; Smg-6 homolog; hSmg5/7a
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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