Recombinant Human Thrombopoietin receptor (MPL), partial, Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 56.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Human Thrombopoietin receptor (MPL), partial

Recombinant Human Thrombopoietin receptor (MPL), partial — Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Purity >90%.

SKU: BCRECP-001181 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-001181
Product DescriptionRecombinant Human Thrombopoietin receptor (MPL) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 26-491aa. [Accession | P40238].
Uniprot No.P40238
Gene NamesMPL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region26-491aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight56.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K. In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein); Golgi apparatus; Cell surface
Tissue Specificity: Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed
Disease Association: Amegakaryocytic thrombocytopenia, congenital, 1 (CAMT1) : An autosomal recessive form of congenital amegakaryocytic thrombocytopenia, a hematologic disorder characterized by severe reduction of megakaryocytes and platelets at birth, and evolving into generalized bone marrow aplasia during childhood. [The disease is caused by variants affecting the gene represented in this entry] | Thrombocythemia 2 (THCYT2) : A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes. [The disease is caused by variants affecting the gene represented in this entry] | Myelofibrosis with myeloid metaplasia (MMM) : A chronic myeloproliferative disorder characterized by replacement of the bone marrow by fibrous tissue, extramedullary hematopoiesis, anemia, leukoerythroblastosis and hepatosplenomegaly. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Thrombopoietin receptor (MPL), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 26-491aa, with N-terminal 6xHis-tagged tag, molecular weight 56.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K (PubMed:15899890, PubMed:37633268). In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production

Subcellular Location

Cell membrane (Single-pass type I membrane protein); Golgi apparatus; Cell surface

Disease Association

Amegakaryocytic thrombocytopenia, congenital, 1 (CAMT1) : An autosomal recessive form of congenital amegakaryocytic thrombocytopenia, a hematologic disorder characterized by severe reduction of megakaryocytes and platelets at birth, and evolving into generalized bone marrow aplasia during childhood. [The disease is caused by variants affecting the gene represented in this entry] | Thrombocythemia 2 (THCYT2) : A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes. [The disease is caused by variants affecting the gene represented in this entry] | Myelofibrosis with myeloid metaplasia (MMM) : A chronic myeloproliferative disorder characterized by replacement of the bone marrow by fibrous tissue, extramedullary hematopoiesis, anemia, leukoerythroblastosis and hepatosplenomegaly. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed

Subunit

Homodimer (PubMed:32029621, PubMed:25538044). Interacts with ATXN2L (PubMed:11784712). Interacts with JAK2 and TYK2; these interactions increase MPL localization to the cell membrane (PubMed:15899890). Interacts with THPO (PubMed:37633268). Interacts with SHIP/INPP5D (PubMed:24607955). Interacts with BTK (PubMed:24607955). Interacts with SYK; this interaction negatively regulates THPO-mediated ERK1/2 signaling (PubMed:24607955)

Gene: MPL  |  Organism: Homo sapiens  |  Synonyms: Myeloproliferative leukemia protein; Proto-oncogene c-Mpl
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top