Recombinant Human Transmembrane protease serine 2 (TMPRSS2) (R255Q), partial (Active), Homo sapiens (Human), Mammalian cell, N-terminal 10xHis-tagged, 46.4 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Transmembrane protease serine 2 (TMPRSS2) (R255Q), partial (Active)

Recombinant Human Transmembrane protease serine 2 (TMPRSS2) (R255Q), partial (Active) — (Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. Purity >90%.

SKU: BCACP-000074 Categories: , ,
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Product SKUBCACP-000074
Product DescriptionRecombinant Human Transmembrane protease serine 2 (TMPRSS2) (R255Q) protein is expressed from Mammalian cell with N-terminal 10xHis-tagged. It contains 106-492aa(R255Q). [Accession | O15393].
Uniprot No.O15393
Gene NamesTMPRSS2
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region106-492aa(R255Q)
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 10xHis-tagged
Molecular weight46.4 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (V367F) at 5 μg/ml can bind human ACE2 , the EC50 is 65.58-90.16 ng/ml.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesTMPRSS2; PRSS10; Transmembrane protease serine 2; Serine protease 10

Function

(Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. After binding to cell-surface disialoside glycans, the viral S protein interacts with the inactive form of TMPRSS2 and inhibits its protease activity.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in several tissues that comprise large populations of epithelial cells with the highest level of transcripts measured in the prostate gland. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland. Coexpressed with ACE2 within lung type II pneumocytes, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells (Ref.21)

Product Specifications

Recombinant Human Transmembrane protease serine 2 (TMPRSS2) (R255Q), partial (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 106-492aa(R255Q), with N-terminal 10xHis-tagged tag, molecular weight 46.4 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

(Microbial infection) Receptor for human coronavirus HKU1-CoV, acts synergistically with disialoside glycans to facilitate the entry of the virus. After binding to cell-surface disialoside glycans, the viral S protein interacts with the inactive form of TMPRSS2 and inhibits its protease activity

Subcellular Location

Secreted

Tissue Specificity

Expressed in several tissues that comprise large populations of epithelial cells with the highest level of transcripts measured in the prostate gland. Expressed in type II pneumocytes in the lung (at protein level). Expressed strongly in small intestine. Also expressed in colon, stomach and salivary gland. Coexpressed with ACE2 within lung type II pneumocytes, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells (Ref.21)

Subunit

The catalytically active form interacts with ACE2

Gene: TMPRSS2  |  Organism: Homo sapiens  |  Synonyms: Serine protease 10
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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