Recombinant Human tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61A (TRMT61A), Homo sapiens (Human), E.coli, N-terminal 6xHis-tagged, 35.5kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Human tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61A (TRMT61A)

Recombinant Human tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61A (TRMT61A) — Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Purity >95%.

SKU: BCRECP-001858 Categories: ,
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Product Specifications

Product SkuBCRECP-001858
Product DescriptionRecombinant Human tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61A (TRMT61A) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-289aa. [Accession | Q96FX7].
Uniprot No.Q96FX7
Gene NamesTRMT61A
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-289aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-tagged
Molecular weight35.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.

Biological Context

Subcellular Location: Nucleus

Product Specifications

Recombinant Human tRNA (adenine (58)-N (1))-methyltransferase catalytic subunit TRMT61A (TRMT61A) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-289aa, with N-terminal 6xHis-tagged tag, molecular weight 35.5kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508). Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29072297, PubMed:29107537)

Subcellular Location

Nucleus

Subunit

Heterotetramer; composed of two copies of TRMT6 and two copies of TRMT61A

Gene: TRMT61A  |  Organism: Homo sapiens  |  Synonyms: mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A; tRNA(m1A58)-methyltransferase subunit TRMT61A
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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