Recombinant Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) (Active), Homo sapiens (Human), Mammalian cell, C-terminal hFc1-Flag-tagged, 73.5 kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) (Active)

Recombinant Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) (Active) — Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Purity >90%.

SKU: BCACP-000101 Categories: ,
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Product SKUBCACP-000101
Product DescriptionRecombinant Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) protein is expressed from Mammalian cell with C-terminal hFc1-Flag-tagged. It contains 22-401aa. [Accession | O00300].
Uniprot No.O00300
Gene NamesTNFRSF11B
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region22-401aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal hFc1-Flag-tagged
Molecular weight73.5 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized NRG1 at 2 μg/ml can bind human ERBB3（CSB-MP007765HU）, the EC50 is 18.24-23.66 ng/ml.;The ED50 as determined by the dose-dependent stimulation of the proliferation of MCF-7 cells is 2.626-7.598 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative Names(Osteoclastogenesis inhibitory factor)(Osteoprotegerin)(OCIF)(OPG)

Function

Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung
Disease Association: Paget disease of bone 5, juvenile-onset (PDB5) : An autosomal recessive, juvenile-onset form of Paget disease, a disorder of bone remodeling characterized by increased bone turnover affecting one or more sites throughout the skeleton, primarily the axial skeleton. Osteoclastic overactivity followed by compensatory osteoblastic activity leads to a structurally disorganized mosaic of bone (woven bone), which is mechanically weaker, larger, less compact, more vascular, and more susceptible to fracture than normal adult lamellar bone. PDB5 clinical manifestations include short stature, progressive long bone deformities, fractures, vertebral collapse, skull enlargement, and hyperostosis with progressive deafness. [The disease is caused by variants affecting the gene represented in this entry]

Product Specifications

Recombinant Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) (Active) is a recombinant protein from Homo sapiens (Human), expressed in Mammalian cell, covering amino acids 22-401aa, with C-terminal hFc1-Flag-tagged tag, molecular weight 73.5 kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized NRG1 at 2 μg/ml can bind human ERBB3（CSB-MP007765HU）, the EC50 is 18.24-23.66 ng/ml.;The ED50 as determined by the dose-dependent stimulation of the proliferation of MCF-7 cells is 2.626-7.598 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis

Subcellular Location

Secreted

Disease Association

Paget disease of bone 5, juvenile-onset (PDB5) : An autosomal recessive, juvenile-onset form of Paget disease, a disorder of bone remodeling characterized by increased bone turnover affecting one or more sites throughout the skeleton, primarily the axial skeleton. Osteoclastic overactivity followed by compensatory osteoblastic activity leads to a structurally disorganized mosaic of bone (woven bone), which is mechanically weaker, larger, less compact, more vascular, and more susceptible to fracture than normal adult lamellar bone. PDB5 clinical manifestations include short stature, progressive long bone deformities, fractures, vertebral collapse, skull enlargement, and hyperostosis with progressive deafness. [The disease is caused by variants affecting the gene represented in this entry]

Tissue Specificity

Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung

Subunit

Homodimer. Interacts with TNFSF10 and TNFSF11

Gene: TNFRSF11B  |  Organism: Homo sapiens  |  Synonyms: Osteoclastogenesis inhibitory factor; Osteoprotegerin
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