Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5), Homo sapiens (Human), E.coli, N-terminal 6xHis-SUMO-tagged, 53.4kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5)

Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) — Protease that specifically cleaves ‘Lys-48’-linked polyubiquitin chains. Purity >90%.

SKU: BCRECP-001888 Categories: ,
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Product Specifications

Product SkuBCRECP-001888
Product DescriptionRecombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-326aa. [Accession | Q9Y5K5].
Uniprot No.Q9Y5K5
Gene NamesUCHL5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-326aa
SpeciesHomo sapiens (Human)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight53.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.

Biological Context

Subcellular Location: Cytoplasm; Nucleus

Product Specifications

Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 1-326aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 53.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1

Subcellular Location

Cytoplasm; Nucleus

Subunit

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB; in vitro ADRM1 and NFRKB compete for interaction with UCHL5. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80

Gene: UCHL5  |  Organism: Homo sapiens  |  Synonyms: Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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