Recombinant Influenza A virus Matrix protein 2 (M) Protein, Influenza A virus (strain A/Puerto Rico/8/1934 H1N1), in vitro E.coli expression system, N-terminal 10xHis-tagged, 12.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Influenza A virus Matrix protein 2 (M) Protein

Recombinant Influenza A virus Matrix protein 2 (M) Protein — Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. Purity >90%.

SKU: BCRECP-00058TM Categories: , ,
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Product Specifications

Uniprot No.P06821
Gene NamesM
PurityGreater than 90% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-97aa
SpeciesInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Tag InfoN-terminal 10xHis-tagged
Molecular weight12.5kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity.

Biological Context

Subcellular Location: Virion membrane; Host apical cell membrane (Single-pass type III membrane protein)

Product Specifications

Recombinant Influenza A virus Matrix protein 2 (M) Protein is a recombinant protein from Influenza A virus (strain A/Puerto Rico/8/1934 H1N1), expressed in in vitro E.coli expression system, covering amino acids 1-97aa, with N-terminal 10xHis-tagged tag, molecular weight 12.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation

Subcellular Location

Virion membrane; Host apical cell membrane (Single-pass type III membrane protein)

Subunit

Homotetramer; composed of two disulfide-linked dimers held together by non-covalent interactions. May interact with matrix protein 1

Gene: M  |  Organism: Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)  |  Synonyms: Proton channel protein M2
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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