Recombinant Influenza A virus Non-structural protein 1 (NS), Influenza A virus (strain A/Alaska/6/1977 H3N2), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 34.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Influenza A virus Non-structural protein 1 (NS)

Recombinant Influenza A virus Non-structural protein 1 (NS) — Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3′-end processing of cellular pre-mRNAs: the 30 kDa cleavage… Purity >90%.

SKU: BCRECP-003910 Categories: , ,
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Product Specifications

Product SkuBCRECP-003910
Product DescriptionRecombinant Influenza A virus Non-structural protein 1 (NS) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-237aa. [Accession | P03494].
Uniprot No.P03494
Gene NamesNS
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-237aa
SpeciesInfluenza A virus (strain A/Alaska/6/1977 H3N2)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight34.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection.

Biological Context

Subcellular Location: Host nucleus; Host cytoplasm

Product Specifications

Recombinant Influenza A virus Non-structural protein 1 (NS) is a recombinant protein from Influenza A virus (strain A/Alaska/6/1977 H3N2), expressed in E.coli, covering amino acids 1-237aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 34.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor/CPSF4 and the poly(A)-binding protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in the host nucleus and are no longer exported to the cytoplasm. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism. Prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated RIGI ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors IRF3 and IRF7. Also binds poly(A) and U6 snRNA. Inhibits the integrated stress response (ISR) in the infected cell by blocking dsRNA binding by EIF2AK2/PKR and further phosphorylation of EIF2S1/EIF-2ALPHA. Stress granule formation is thus inhibited, which allows protein synthesis and viral replication

Subcellular Location

Host nucleus; Host cytoplasm

Subunit

Homodimer. Interacts with host TRIM25 (via coiled coil); this interaction specifically inhibits TRIM25 multimerization and TRIM25-mediated RIGI CARD ubiquitination. Interacts with human EIF2AK2/PKR, CPSF4, IVNS1ABP and PABPN1

Gene: NS  |  Organism: Influenza A virus (strain A/Alaska/6/1977 H3N2)  |  Synonyms: NS1A

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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