Recombinant Influenza A virus Polymerase acidic protein (PA), partial, Influenza A virus (strain A/x-31 H3N2), E.coli, N-terminal 6xHis-tagged, 18.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Influenza A virus Polymerase acidic protein (PA), partial

Recombinant Influenza A virus Polymerase acidic protein (PA), partial — Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. Purity >90%.

SKU: BCRECP-004002 Categories: , ,
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Product Specifications

Product SkuBCRECP-004002
Product DescriptionRecombinant Influenza A virus Polymerase acidic protein (PA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 124-247aa. [Accession | Q9IQ47].
Uniprot No.Q9IQ47
Gene NamesPA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region124-247aa
SpeciesInfluenza A virus (strain A/x-31 H3N2)
Tag InfoN-terminal 6xHis-tagged
Molecular weight18.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs.

Biological Context

Subcellular Location: Host cytoplasm; Host nucleus

Product Specifications

Recombinant Influenza A virus Polymerase acidic protein (PA), partial is a recombinant protein from Influenza A virus (strain A/x-31 H3N2), expressed in E.coli, covering amino acids 124-247aa, with N-terminal 6xHis-tagged tag, molecular weight 18.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity

Subcellular Location

Host cytoplasm; Host nucleus

Subunit

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus)

Gene: PA  |  Organism: Influenza A virus (strain A/X-31 H3N2)  |  Synonyms: RNA-directed RNA polymerase subunit P2

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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