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Recombinant Influenza A virus Polymerase acidic protein (PA), partial

Recombinant Influenza A virus Polymerase acidic protein (PA), partial — Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. Purity >90%.

Product Specifications

Product SkuBCRECP-004002
Product DescriptionRecombinant Influenza A virus Polymerase acidic protein (PA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 124-247aa. [Accession | Q9IQ47].
Uniprot No.Q9IQ47
Gene NamesPA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region124-247aa
SpeciesInfluenza A virus (strain A/x-31 H3N2)
Tag InfoN-terminal 6xHis-tagged
Molecular weight18.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs.

Biological Context

Subcellular Location: Host cytoplasm; Host nucleus

Product Specifications

Recombinant Influenza A virus Polymerase acidic protein (PA), partial is a recombinant protein from Influenza A virus (strain A/x-31 H3N2), expressed in E.coli, covering amino acids 124-247aa, with N-terminal 6xHis-tagged tag, molecular weight 18.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity

Subcellular Location

Host cytoplasm; Host nucleus

Subunit

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus)

Gene: PA  |  Organism: Influenza A virus (strain A/X-31 H3N2)  |  Synonyms: RNA-directed RNA polymerase subunit P2

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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