Recombinant JC polyomavirus Small t antigen, JC polyomavirus (JCPyV) (JCV), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant JC polyomavirus Small t antigen

Recombinant JC polyomavirus Small t antigen — Promotes efficient viral genome replication by modulating several host signaling pathways including transport network, interferon production or cell cycle progression. Purity >85%.

SKU: BCRECP-003658 Categories: , ,
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Product Specifications

Product SkuBCRECP-003658
Product DescriptionRecombinant JC polyomavirus Small t antigen Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-172aa. [Accession | P03083].
Uniprot No.P03083
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-172aa
SpeciesJC polyomavirus (JCPyV) (JCV)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Promotes efficient viral genome replication by modulating several host signaling pathways including transport network, interferon production or cell cycle progression. Inhibits host PP2A phosphatase activity and thereby prevents agnoprotein dephosphorylation. Inactivation of PP2A also results in the transactivation of cyclin A and cyclin D1 promoters.

Biological Context

Subcellular Location: Host cytoplasm; Host nucleus

Product Specifications

Recombinant JC polyomavirus Small t antigen is a recombinant protein from JC polyomavirus (JCPyV) (JCV), expressed in E.coli, covering amino acids 1-172aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Promotes efficient viral genome replication by modulating several host signaling pathways including transport network, interferon production or cell cycle progression (PubMed:20485545, PubMed:33092197, PubMed:33849980). Inhibits host PP2A phosphatase activity and thereby prevents agnoprotein dephosphorylation (PubMed:18353419, PubMed:20485545). Inactivation of PP2A also results in the transactivation of cyclin A and cyclin D1 promoters (By similarity). In addition, antagonizes the RIGI-mediated IFN response through interaction with E3 ligase TRIM25 leading to the inhibition of 'Lys-63'-linked ubiquitination of RIGI. Inhibits nucleotide excision repair (NER) pathway which leads to DNA strand breaks during DNA replication and micronuclei formation (PubMed:25744060)

Subcellular Location

Host cytoplasm; Host nucleus

Subunit

Interacts with host PPP2R1A; the interaction inhibits PP2A activity (PubMed:20485545). Interacts with agnoprotein; this interaction prevents agnoprotein dephosphorylation by host PP2A (PubMed:18353419). Interacts with host RBL1 and RBL2 (PubMed:20485545). Interacts with SMARCA5 (PubMed:33092197). Interacts with SDHB (PubMed:33092197)

Organism: JC polyomavirus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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