Recombinant Lactobacillus reuteri ATP synthase subunit beta (atpD), Lactobacillus reuteri (strain DSM 20016), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 59.1kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Lactobacillus reuteri ATP synthase subunit beta (atpD)

Recombinant Lactobacillus reuteri ATP synthase subunit beta (atpD) — Produces ATP from ADP in the presence of a proton gradient across the membrane. Purity >85%.

SKU: BCRECP-002164 Categories: ,
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Product Specifications

Product SkuBCRECP-002164
Product DescriptionRecombinant Lactobacillus reuteri ATP synthase subunit beta (atpD) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-475aa. [Accession | A5VIR1].
Uniprot No.A5VIR1
Gene NamesatpD
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-475aa
SpeciesLactobacillus reuteri (strain DSM 20016)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight59.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.

Biological Context

Subcellular Location: Cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Lactobacillus reuteri ATP synthase subunit beta (atpD) is a recombinant protein from Lactobacillus reuteri (strain DSM 20016), expressed in E.coli, covering amino acids 1-475aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 59.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits

Subcellular Location

Cell membrane (Peripheral membrane protein)

Subunit

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains

Gene: atpD  |  Organism: Limosilactobacillus reuteri (strain DSM 20016)  |  Synonyms: ATP synthase F1 sector subunit beta; F-ATPase subunit beta

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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