Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial, Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial

Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial — RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. Purity >90%.

SKU: BCRECP-003227 Categories: ,
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Product Specifications

Product SkuBCRECP-003227
Product DescriptionRecombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 628-812aa. [Accession | P35262].
Uniprot No.P35262
Gene NamesL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region628-812aa
SpeciesLake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration.

Biological Context

Subcellular Location: Host cytoplasm; Virion

Product Specifications

Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial is a recombinant protein from Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)), expressed in E.coli, covering amino acids 628-812aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated

Subcellular Location

Host cytoplasm; Virion

Subunit

Interacts with VP35; this complex constitutes a functional polymerase complex

Gene: L  |  Organism: Lake Victoria marburgvirus (strain Popp-67)  |  Synonyms: Large structural protein; Replicase; Transcriptase

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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