Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial, Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial

Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial — RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. Purity >90%.

SKU: BCRECP-003227 Categories: ,
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Product Specifications

Product SkuBCRECP-003227
Product DescriptionRecombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 628-812aa. [Accession | P35262].
Uniprot No.P35262
Gene NamesL
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region628-812aa
SpeciesLake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967))
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration.

Biological Context

Subcellular Location: Host cytoplasm; Virion

Product Specifications

Recombinant Lake Victoria marburgvirus RNA-directed RNA polymerase L (L), partial is a recombinant protein from Lake Victoria marburgvirus (strain Popp-67) (MARV) (Marburg virus (strain West Germany/Popp/1967)), expressed in E.coli, covering amino acids 628-812aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated

Subcellular Location

Host cytoplasm; Virion

Subunit

Interacts with VP35; this complex constitutes a functional polymerase complex

Gene: L  |  Organism: Lake Victoria marburgvirus (strain Popp-67)  |  Synonyms: Large structural protein; Replicase; Transcriptase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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