Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial, Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus), E.coli, N-terminal 6xHis-tagged, 24.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial

Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial — RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. Purity >85%.

SKU: BCRECP-003225 Categories: , , ,
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Product Specifications

Product SkuBCRECP-003225
Product DescriptionRecombinant Zaire ebolavirus RNA-directed RNA polymerase L (L) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 625-809aa. [Accession | Q05318].
Uniprot No.Q05318
Gene NamesL
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region625-809aa
SpeciesZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Tag InfoN-terminal 6xHis-tagged
Molecular weight24.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration.

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial is a recombinant protein from Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus), expressed in E.coli, covering amino acids 625-809aa, with N-terminal 6xHis-tagged tag, molecular weight 24.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated

Subcellular Location

Virion; Host cytoplasm

Subunit

Forms homooligomers. Interacts with VP35; this complex constitutes a functional polymerase complex

Gene: L  |  Organism: Zaire ebolavirus (strain Mayinga-76)  |  Synonyms: Large structural protein; Replicase; Transcriptase
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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