Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial, Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus), E.coli, N-terminal 6xHis-tagged, 24.9kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial

Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial — RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. Purity >85%.

SKU: BCRECP-003225 Categories: , ,
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Product Specifications

Product SkuBCRECP-003225
Product DescriptionRecombinant Zaire ebolavirus RNA-directed RNA polymerase L (L) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 625-809aa. [Accession | Q05318].
Uniprot No.Q05318
Gene NamesL
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region625-809aa
SpeciesZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Tag InfoN-terminal 6xHis-tagged
Molecular weight24.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration.

Biological Context

Subcellular Location: Virion; Host cytoplasm

Product Specifications

Recombinant Zaire ebolavirus RNA-directed RNA polymerase L (L), partial is a recombinant protein from Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus), expressed in E.coli, covering amino acids 625-809aa, with N-terminal 6xHis-tagged tag, molecular weight 24.9kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Related products

Protein Biology

Function

RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated

Subcellular Location

Virion; Host cytoplasm

Subunit

Forms homooligomers. Interacts with VP35; this complex constitutes a functional polymerase complex

Gene: L  |  Organism: Zaire ebolavirus (strain Mayinga-76)  |  Synonyms: Large structural protein; Replicase; Transcriptase
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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