Recombinant Larimichthys crocea Hepcidin (hamp), Larimichthys crocea (Large yellow croaker) (Pseudosciaena crocea), E.coli, Tag-Free, 2.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Larimichthys crocea Hepcidin (hamp)

Recombinant Larimichthys crocea Hepcidin (hamp) — Has very strong antibacterial activity against the marine Gram-negative bacteria V. Purity >90%.

SKU: BCRECP-002965 Category:
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Product Specifications

Product SkuBCRECP-002965
Product DescriptionRecombinant Larimichthys crocea Hepcidin (hamp) Protein is expressed from E.coli with Tag-Free. It contains 65-85aa. [Accession | A1Z0M0].
Uniprot No.A1Z0M0
Gene Nameshamp
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region65-85aa
SpeciesLarimichthys crocea (Large yellow croaker) (Pseudosciaena crocea)
Tag InfoTag-Free
Molecular weight2.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Has very strong antibacterial activity against the marine Gram-negative bacteria V.alginolyticus (MIC=24 uM), V.fluvialis, V.harveyis (MIC=12 uM) and V.parahaemolyticus (MIC=6 uM). Has antibacterial activity against the Gram-negative bacteria A.hydrophila (MIC=6 uM), E.coli (MIC=24 uM), and E.coli BL21(DE3)plysS (MIC=6 uM), and the Gram-positive bacteria B.cereus (MIC=24 uM), B.subtilis (MIC=6 uM), C.glutamicum (MIC=3 uM), M.luteus (MIC=3 uM), M.lysodeikticus, S.aureus (MIC=6 uM) and S.epidermis (MIC=12 uM). Possesses antifungal activity against A.niger (MIC=24 uM), F.graminearum (MIC24 uM) and F.solani (MIC=24 uM), but lacks antifungal activity against the yeasts P.pastoris GS115 and C.albicans.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in all tissues tested, with highest levels of expression in kidney and lowest levels in liver. Intra-peritoneal injection of lipopolysaccharide results in increased expression in heart, spleen and stomach, but not in kidney or liver

Product Specifications

Recombinant Larimichthys crocea Hepcidin (hamp) is a recombinant protein from Larimichthys crocea (Large yellow croaker) (Pseudosciaena crocea), expressed in E.coli, covering amino acids 65-85aa, with Tag-Free tag, molecular weight 2.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Has very strong antibacterial activity against the marine Gram-negative bacteria V.alginolyticus (MIC=24 uM), V.fluvialis, V.harveyis (MIC=12 uM) and V.parahaemolyticus (MIC=6 uM). Has antibacterial activity against the Gram-negative bacteria A.hydrophila (MIC=6 uM), E.coli (MIC=24 uM), and E.coli BL21(DE3)plysS (MIC=6 uM), and the Gram-positive bacteria B.cereus (MIC=24 uM), B.subtilis (MIC=6 uM), C.glutamicum (MIC=3 uM), M.luteus (MIC=3 uM), M.lysodeikticus, S.aureus (MIC=6 uM) and S.epidermis (MIC=12 uM). Possesses antifungal activity against A.niger (MIC=24 uM), F.graminearum (MIC24 uM) and F.solani (MIC=24 uM), but lacks antifungal activity against the yeasts P.pastoris GS115 and C.albicans

Subcellular Location

Secreted

Tissue Specificity

Expressed in all tissues tested, with highest levels of expression in kidney and lowest levels in liver. Intra-peritoneal injection of lipopolysaccharide results in increased expression in heart, spleen and stomach, but not in kidney or liver

Subunit

Monomer

Gene: hamp  |  Organism: Larimichthys crocea

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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