Recombinant Mouse Hepcidin (Hamp), partial, Mus musculus (Mouse), E.coli, N-terminal GST-tagged, 29.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Hepcidin (Hamp), partial

Recombinant Mouse Hepcidin (Hamp), partial — Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Purity >90%.

SKU: BCRECP-002967 Category:
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Product Specifications

Product SkuBCRECP-002967
Product DescriptionRecombinant Mouse Hepcidin (Hamp) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 59-83aa. [Accession | Q9EQ21].
Uniprot No.Q9EQ21
Gene NamesHamp
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region59-83aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal GST-tagged
Molecular weight29.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Highly expressed in the liver and to a much lesser extent in the heart. Secreted in blood

Product Specifications

Recombinant Mouse Hepcidin (Hamp), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 59-83aa, with N-terminal GST-tagged tag, molecular weight 29.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes

Subcellular Location

Secreted

Tissue Specificity

Highly expressed in the liver and to a much lesser extent in the heart. Secreted in blood (PubMed:15124018)

Subunit

Interacts with SLC40A1; this interaction promotes SLC40A1 rapid ubiquitination

Gene: Hamp  |  Organism: Mus musculus
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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