Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), partial, Lymphocytic choriomeningitis virus (strain WE) (LCMV), E.coli, C-terminal 6xHis-tagged, 24.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), partial

Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), partial — Forms the virion spikes together with glycoprotein G1. Purity >90%.

SKU: BCRECP-002897 Categories: ,
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Product Specifications

Product SkuBCRECP-002897
Product DescriptionRecombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 59-265aa. [Accession | P07399].
Uniprot No.P07399
Gene NamesGPC
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region59-265aa
SpeciesLymphocytic choriomeningitis virus (strain WE) (LCMV)
Tag InfoC-terminal 6xHis-tagged
Molecular weight24.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm.

Biological Context

Subcellular Location: Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Product Specifications

Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), partial is a recombinant protein from Lymphocytic choriomeningitis virus (strain WE) (LCMV), expressed in E.coli, covering amino acids 59-265aa, with C-terminal 6xHis-tagged tag, molecular weight 24.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced by acidification

Subcellular Location

Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Subunit

Homotrimer. Interacts with the stable signal peptide. In pre-fusion state, G2 homotrimers bind G1 homotrimers via ionic interactions (PubMed:27111888). Part of the GP complex (GP-C) together with glycoprotein G1 and the stable signal peptide (By similarity). Acidification in the endosome triggers rearrangements, which ultimately leads to a 6 helix bundle formed by the two heptad repeat domains (HR1 and HR2) in post-fusion state (PubMed:22123988). The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding (By similarity)

Gene: GPC  |  Organism: Lymphocytic choriomeningitis virus (strain WE)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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