Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), Partial, Lymphocytic choriomeningitis virus (strain Armstrong) (LCMV), E.coli, C-terminal 6xHis-tagged, 33.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), Partial

Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), Partial — Forms the virion spikes together with glycoprotein G1. Purity >90%.

SKU: BCRECP-002895 Categories: ,
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Product Specifications

Product SkuBCRECP-002895
Product DescriptionRecombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 266-498aa. [Accession | P09991].
Uniprot No.P09991
Gene NamesGPC
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region266-498aa
SpeciesLymphocytic choriomeningitis virus (strain Armstrong) (LCMV)
Tag InfoC-terminal 6xHis-tagged
Molecular weight33.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm.

Biological Context

Subcellular Location: Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Product Specifications

Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), Partial is a recombinant protein from Lymphocytic choriomeningitis virus (strain Armstrong) (LCMV), expressed in E.coli, covering amino acids 266-498aa, with C-terminal 6xHis-tagged tag, molecular weight 33.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm (PubMed:16731928). Membrane fusion is mediated by irreversible conformational changes induced by acidification

Subcellular Location

Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Subunit

Homotrimer (PubMed:16731928). Interacts with the stable signal peptide. In pre-fusion state, G2 homotrimers bind G1 homotrimers via ionic interactions. Part of the GP complex (GP-C) together with glycoprotein G1 and the stable signal peptide. Acidification in the endosome triggers rearrangements, which ultimately leads to a 6 helix bundle formed by the two heptad repeat domains (HR1 and HR2) in post-fusion state. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding (PubMed:17581989)

Gene: GPC  |  Organism: Lymphocytic choriomeningitis virus (strain Armstrong)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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