Recombinant Lymphocytic choriomeningitis virus Pre-glycoprotein polyprotein GP complex (GPC), partial Protein

Function

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm (PubMed:16731928). Membrane fusion is mediated by irreversible conformational changes induced by acidification

Subcellular Location

Virion membrane (Single-pass membrane protein); Host endoplasmic reticulum membrane (Single-pass membrane protein); Host Golgi apparatus membrane (Single-pass membrane protein); Host cell membrane (Single-pass membrane protein)

Subunit

Homotrimer (PubMed:16731928). Interacts with the stable signal peptide. In pre-fusion state, G2 homotrimers bind G1 homotrimers via ionic interactions. Part of the GP complex (GP-C) together with glycoprotein G1 and the stable signal peptide. Acidification in the endosome triggers rearrangements, which ultimately leads to a 6 helix bundle formed by the two heptad repeat domains (HR1 and HR2) in post-fusion state. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding (PubMed:17581989)

• Southern P.J. et al. Molecular characterization of the genomic S RNA segment from lymphocytic choriomeningitis virus. — Virology (1987)
• Salvato M. et al. Virus-lymphocyte interactions. IV. Molecular characterization of LCMV Armstrong (CTL+) small genomic segment and that of its variant, Clone 13 (CTL-). — Virology (1988)
• Grande-Perez A. et al. Mutagenesis-induced, large fitness variations with an invariant arenavirus consensus genomic nucleotide sequence. — J. Virol. (2005)
• Flatz L. et al. Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA. — Proc. Natl. Acad. Sci. U.S.A. (2006)
• Di Simone C. et al. Acidic pH triggers LCMV membrane fusion activity and conformational change in the glycoprotein spike. — Virology (1994)
• Cao W. et al. Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus. — Science (1998)
• Eschli B. et al. Identification of an N-terminal trimeric coiled-coil core within arenavirus glycoprotein 2 permits assignment to class I viral fusion proteins. — J. Virol. (2006)
• Saunders A.A. et al. Mapping the landscape of the lymphocytic choriomeningitis virus stable signal peptide reveals novel functional domains. — J. Virol. (2007)