Recombinant Macaca fascicularis Legumain (LGMN) (Active), Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), Mammalian cell, C-terminal 10xHis-tagged, 49.0 kDa, purity >95% as determined by SDS-PAGE.

Recombinant Macaca fascicularis Legumain (LGMN) (Active)

Recombinant Macaca fascicularis Legumain (LGMN) (Active) — Has a strict specificity for hydrolysis of asparaginyl bonds. Purity >95%.

SKU: BCACP-000588 Category:
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Product SKUBCACP-000588
Product DescriptionRecombinant Cynomolgus monkey Legumain (LGMN) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 18-433aa. [Accession | Q4R4T8].
Uniprot No.Q4R4T8
Gene NamesLGMN
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region18-433aa
SpeciesMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Tag InfoC-terminal 10xHis-tagged
Molecular weight49.0 kDa
ActivityMeasured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >400 pmol/min/µg.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesLegumain; EC 3.4.22.34; Asparaginyl endopeptidase; Protease, cysteine 1

Function

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Biological Context

Subcellular Location: Lysosome

Product Specifications

Recombinant Macaca fascicularis Legumain (LGMN) (Active) is a recombinant protein from Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey), expressed in Mammalian cell, covering amino acids 18-433aa, with C-terminal 10xHis-tagged tag, molecular weight 49.0 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is >400 pmol/min/µg.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (By similarity). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity)

Subcellular Location

Lysosome

Subunit

Homodimer before autocatalytic removal of the propeptide. Monomer after autocatalytic processing. May interact with integrins

Gene: LGMN  |  Organism: Macaca fascicularis  |  Synonyms: Asparaginyl endopeptidase; Protease, cysteine 1

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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