Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial, Macrococcus caseolyticus (strain JCSC5402), E.coli, N-terminal 6xHis-tagged, 45.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial

Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial — Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration. Purity >85%.

SKU: BCRECP-003366 Category:
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Product Specifications

Product SkuBCRECP-003366
Product DescriptionRecombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-367aa. [Accession | B9E972].
Uniprot No.B9E972
Gene NamesMCCL_0076
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-367aa
SpeciesMacrococcus caseolyticus (strain JCSC5402)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration. Cannot catalyze the reverse reaction. Is not active with saturated fatty acids and trans-, cis-5-, cis-6-, cis-8-, cis-11-, cis-13-, cis-14-, and cis-15-double bond unsaturated fatty acids as substrate; is only active on cis-9- and/or cis-12-double bond of unsaturated fatty acids without any trans-configurations, producing 10-hydroxy and, to a lesser extent, 10,13-dihydroxy fatty acids.

Biological Context

Pathway: Lipid metabolism; fatty acid metabolism

Product Specifications

Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial is a recombinant protein from Macrococcus caseolyticus (strain JCSC5402), expressed in E.coli, covering amino acids 1-367aa, with N-terminal 6xHis-tagged tag, molecular weight 45.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration. Cannot catalyze the reverse reaction. Is not active with saturated fatty acids and trans-, cis-5-, cis-6-, cis-8-, cis-11-, cis-13-, cis-14-, and cis-15-double bond unsaturated fatty acids as substrate; is only active on cis-9- and/or cis-12-double bond of unsaturated fatty acids without any trans-configurations, producing 10-hydroxy and, to a lesser extent, 10,13-dihydroxy fatty acids. The hydration of unsaturated fatty acids is suggested to be a detoxification mechanism and a survival strategy for living in fatty acid-rich environments

Subunit

Homodimer

Pathway

Lipid metabolism; fatty acid metabolism

Organism: Macrococcoides caseolyticum (strain JCSC5402)  |  Synonyms: Fatty acid double bond hydratase; Fatty acid hydratase

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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