Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial, Macrococcus caseolyticus (strain JCSC5402), E.coli, N-terminal 6xHis-tagged, 45.5kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial

Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial — Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration. Purity >85%.

SKU: BCRECP-003366 Categories: ,
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Product Specifications

Product SkuBCRECP-003366
Product DescriptionRecombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-367aa. [Accession | B9E972].
Uniprot No.B9E972
Gene NamesMCCL_0076
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-367aa
SpeciesMacrococcus caseolyticus (strain JCSC5402)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration. Cannot catalyze the reverse reaction. Is not active with saturated fatty acids and trans-, cis-5-, cis-6-, cis-8-, cis-11-, cis-13-, cis-14-, and cis-15-double bond unsaturated fatty acids as substrate; is only active on cis-9- and/or cis-12-double bond of unsaturated fatty acids without any trans-configurations, producing 10-hydroxy and, to a lesser extent, 10,13-dihydroxy fatty acids.

Biological Context

Pathway: Lipid metabolism; fatty acid metabolism

Product Specifications

Recombinant Macrococcus caseolyticus Oleate hydratase (MCCL_0076), partial is a recombinant protein from Macrococcus caseolyticus (strain JCSC5402), expressed in E.coli, covering amino acids 1-367aa, with N-terminal 6xHis-tagged tag, molecular weight 45.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Catalyzes the hydration of oleate at its cis-9-double bond to yield 10-hydroxyoctadecanoate, probably in the (R) configuration. Cannot catalyze the reverse reaction. Is not active with saturated fatty acids and trans-, cis-5-, cis-6-, cis-8-, cis-11-, cis-13-, cis-14-, and cis-15-double bond unsaturated fatty acids as substrate; is only active on cis-9- and/or cis-12-double bond of unsaturated fatty acids without any trans-configurations, producing 10-hydroxy and, to a lesser extent, 10,13-dihydroxy fatty acids. The hydration of unsaturated fatty acids is suggested to be a detoxification mechanism and a survival strategy for living in fatty acid-rich environments

Subunit

Homodimer

Pathway

Lipid metabolism; fatty acid metabolism

Organism: Macrococcoides caseolyticum (strain JCSC5402)  |  Synonyms: Fatty acid double bond hydratase; Fatty acid hydratase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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