Recombinant Mannheimia haemolytica Leukotoxin (lktA), partial, Mannheimia haemolytica (Pasteurella haemolytica), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 33.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mannheimia haemolytica Leukotoxin (lktA), partial

Recombinant Mannheimia haemolytica Leukotoxin (lktA), partial — Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. Purity >90%.

SKU: BCRECP-003269 Category:
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Product Specifications

Product SkuBCRECP-003269
Product DescriptionRecombinant Mannheimia haemolytica Leukotoxin (lktA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 712-954aa. [Accession | P0C082].
Uniprot No.P0C082
Gene NameslktA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region712-954aa
SpeciesMannheimia haemolytica (Pasteurella haemolytica)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight33.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca(2+) and lysis of the host cell. This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis.

Biological Context

Subcellular Location: Secreted; Host cell membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Mannheimia haemolytica Leukotoxin (lktA), partial is a recombinant protein from Mannheimia haemolytica (Pasteurella haemolytica), expressed in E.coli, covering amino acids 712-954aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 33.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca(2+) and lysis of the host cell (By similarity). This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis. It also has weak hemolytic activity

Subcellular Location

Secreted; Host cell membrane (Multi-pass membrane protein)

Gene: lktA  |  Organism: Mannheimia haemolytica

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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