Recombinant Mesobuthus martensii Beta-insect depressant toxin BmKITa, partial, Mesobuthus martensii (Manchurian scorpion) (Buthus martensii), E.coli, N-terminal 10xHis-tagged, 12.8kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mesobuthus martensii Beta-insect depressant toxin BmKITa, partial

Recombinant Mesobuthus martensii Beta-insect depressant toxin BmKITa, partial — Depressant insect beta-toxins cause a transient contraction paralysis followed by a slow flaccid paralysis. Purity >90%.

SKU: BCRECP-003770 Category:
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Product Specifications

Product SkuBCRECP-003770
Product DescriptionRecombinant Mesobuthus martensii Beta-insect depressant toxin BmKITa, partial Protein is expressed from E.coli with N-terminal 10xHis-tagged. It contains 22-82aa. [Accession | Q9XY87].
Uniprot No.Q9XY87
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region22-82aa
SpeciesMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Tag InfoN-terminal 10xHis-tagged
Molecular weight12.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Depressant insect beta-toxins cause a transient contraction paralysis followed by a slow flaccid paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin also displays an evident analgesic effect but is devoid of any toxicity on mice.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom gland

Product Specifications

Recombinant Mesobuthus martensii Beta-insect depressant toxin BmKITa, partial is a recombinant protein from Mesobuthus martensii (Manchurian scorpion) (Buthus martensii), expressed in E.coli, covering amino acids 22-82aa, with N-terminal 10xHis-tagged tag, molecular weight 12.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Depressant insect beta-toxins cause a transient contraction paralysis followed by a slow flaccid paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin also displays an evident analgesic effect but is devoid of any toxicity on mice

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom gland

Organism: Olivierus martensii  |  Synonyms: BmK dITAP3

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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