Recombinant Mesocricetus auratus CD27 antigen (Cd27), partial, Mesocricetus auratus (Golden hamster), E.coli, N-terminal 6xHis-tagged and C-terminal Strep II-tagged, 20.5kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mesocricetus auratus CD27 antigen (Cd27), partial

Recombinant Mesocricetus auratus CD27 antigen (Cd27), partial — Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. Purity >90%.

SKU: BCRECP-002315 Categories: ,
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Product Specifications

Product SkuBCRECP-002315
Product DescriptionRecombinant Mesocricetus auratus CD27 antigen (Cd27) Protein is expressed from E.coli with N-terminal 6xHis-tagged and C-terminal Strep II-tagged. It contains 21-187aa. [Accession | A0A3Q0DDP5].
Uniprot No.A0A3Q0DDP5
Gene NamesCd27
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region21-187aa
SpeciesMesocricetus auratus (Golden hamster)
Tag InfoN-terminal 6xHis-tagged and C-terminal Strep II-tagged
Molecular weight20.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Mesocricetus auratus CD27 antigen (Cd27), partial is a recombinant protein from Mesocricetus auratus (Golden hamster), expressed in E.coli, covering amino acids 21-187aa, with N-terminal 6xHis-tagged and C-terminal Strep II-tagged tag, molecular weight 20.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Homodimer. Interacts with SIVA1; may play a role in apoptosis through association with SIVA1. Interacts with TRAF2. Interacts ith PTPN6

Gene: Cd27  |  Organism: Mesocricetus auratus  |  Synonyms: CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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