Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 45.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1)

Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1) — Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. Purity >90%.

SKU: BCRECP-002969 Categories: ,
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Product Specifications

Product SkuBCRECP-002969
Product DescriptionRecombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-370aa. [Accession | Q9WU19].
Uniprot No.Q9WU19
Gene NamesHao1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-370aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasTags & Cell Markers

Function

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones. Can also catalyze the oxidation glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate.

Biological Context

Subcellular Location: Peroxisome matrix
Tissue Specificity: Liver
Pathway: Amino-acid biosynthesis; glycine biosynthesis

Product Specifications

Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-370aa, with N-terminal 6xHis-tagged tag, molecular weight 45.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:9891009). The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (By similarity). Can also catalyze the oxidation glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate (By similarity). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:9891009)

Subcellular Location

Peroxisome matrix

Tissue Specificity

Liver

Subunit

Homotetramer

Pathway

Amino-acid biosynthesis; glycine biosynthesis

Gene: Hao1  |  Organism: Mus musculus  |  Synonyms: Glycolate oxidase; Glyoxylate oxidase

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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