Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1), Mus musculus (Mouse), E.coli, N-terminal 6xHis-tagged, 45.0kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1)

Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1) — Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. Purity >90%.

SKU: BCRECP-002969 Categories: ,
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Product Specifications

Product SkuBCRECP-002969
Product DescriptionRecombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-370aa. [Accession | Q9WU19].
Uniprot No.Q9WU19
Gene NamesHao1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-370aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasTags & Cell Markers

Function

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate. The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones. Can also catalyze the oxidation glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate.

Biological Context

Subcellular Location: Peroxisome matrix
Tissue Specificity: Liver
Pathway: Amino-acid biosynthesis; glycine biosynthesis

Product Specifications

Recombinant Mouse 2-Hydroxyacid oxidase 1 (Hao1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-370aa, with N-terminal 6xHis-tagged tag, molecular weight 45.0kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Broad substrate specificity (S)-2-hydroxy-acid oxidase that preferentially oxidizes glycolate (PubMed:9891009). The glyoxylate produced by the oxidation of glycolate can then be utilized by alanine-glyoxylate aminotransferase for the peroxisomal synthesis of glycine; this pathway appears to be an important step for the detoxification of glyoxylate which, if allowed to accumulate, may be metabolized to oxalate with formation of kidney stones (By similarity). Can also catalyze the oxidation glyoxylate, and long chain hydroxyacids such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate (By similarity). Active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), but O2 is believed to be the physiological electron acceptor, leading to the production of H2O2 (PubMed:9891009)

Subcellular Location

Peroxisome matrix

Tissue Specificity

Liver

Subunit

Homotetramer

Pathway

Amino-acid biosynthesis; glycine biosynthesis

Gene: Hao1  |  Organism: Mus musculus  |  Synonyms: Glycolate oxidase; Glyoxylate oxidase

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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